About the project
The current aim of the project is to make a multicellular model of cardiac tissue, in which all cells are derived from iPSCs. In many cardiac diseases, it is not enough to investigate cardiomyocyte dysfunction: Endothelial cells and fibroblasts have major implications in cardiac function, both on macroscopic level and in intercellular crosstalk. To meet these demands, we present a novel dual-chambered chip, optimized for multicellular cell seeding combined with physiological microfluidics. By deriving endothelium, fibroblasts and cardiomyocytes from one hiPSC-line, we can create patient-specific Heart-on-Chip, for advanced disease modeling, drug-toxicity testing and pharmaceutical screening.
The first priorities for the use of this model are to validate the endothelial barrier and cardiomyocyte function, and characterize known cell-cell signaling pathways between the three cell-types. The chip-holder allows for live-imaging without the need of disassembly. Therefore, a broad range of read-outs (imaging data, qPCR, medium sampling) will enable thorough qualitative and quantitative analysis of the cellular actuators in the device.